No.
AirFly SE and AirFly Duo transmit audio wirelessly from any 3.5mm headphone jack to your favorite wireless headphones or AirPods. However, they do not receive audio, so they cannot be used in any function that would require sending audio back to the 3.5mm-connected device, such as calling or chatting back and forth using microphone-enabled headphones or a gaming system.
And although AirFly Pro will transmit and receive audio, it will not switch between the two automatically, so it cannot be used for telephone conversations.